Correlations among homocysteine, cardiac troponin I and cardiac enzymes in different ages of clinically healthy male dromedary camels

Information regarding serum biochemical profile can reflect cardiovascular performance in animals. Although studies have evaluated the inter-relationship among cardiovascular biomarkers in animals and human beings, there are no reports of such a probable relationship in camelids. The aim of the present study was to provide data on the correlations among cardiovascular biomarkers in different ages of clinically healthy male dromedary camels to provide a basis for assessing cardiac muscle healthiness in this species. Thirty clinically healthy dromedary camels [Camelus dromedarius] were selected and divided into four age groups including 1-3 [n=7], 4-6 [n=7], 7-9 [n=8], and above 10 [n=8] years old. Blood samples were collected and sera were separated. Serum concentrations of homocysteine [Hcy], cardiac troponin I [cTnI], creatine kinase-myocardial specific isoenzymes [CK-MB], lactate dehydrogenase [LDH], alanine aminotransferase [ALT] and aspartate aminotransferase [AST] were evaluated. The results of the present study showed that there were significant correlations among cTnI and CK-MB [r=-0.853; p=0.015] and Hcy [r=0.916; p=0.004] in the 4 to 6-years-old group of clinically healthy male dromedary camels. LDH was significantly correlated with CK-MB in the 7 to 9-year-old group [r=-0.710; p=0.045]. There were no significant correlations among different factors of 1-3 and above 10-year-old groups [p>0.05]. The data provided here is the first report on cardiac health assessment parameters in dromedary camels. Moreover, the data is valuable in camel racing clubs, when an overall cardiac health and fitness is to be assessed. The correlation reported here might also be helpful for easier analysis of cardiac health status in dromedary camels. The data may be useful for assessing suspected cases of myocardial diseases and its changes maybe of prognostic value

[ effect of orientation tour with angiography procedure on anxiety and satisfaction of patients undergoing coronary angiography]

Recently, coronary angiography has become the largest interventional diagnostic test in cardiac patients worldwide having large effects on anxiety and satisfaction of patients in treatment and care. This study aimed to study the effect of orientation tour on anxiety and satisfaction of patient candidates for coronary angiography procedures. In this randomized control trial study, 148 patients were recruited by random allocation. Patients were divided into two groups with block randomization. Patients in Intervention group, in addition to routine training, orientation tour in the angiography unit was taken too. Anxiety was measured in all patients during admission, before surgery and discharge time; the satisfaction was measured in discharge time too. Collection instruments were according to demographic data, Spielberger state trait anxiety inventory and the Visual Analogue Scale. The Information was obtained by t-test, chi-square and repeated measured were analyzed by SPSS. After the orientation tour, anxiety scores significantly in the intervention group [39.5] compared with the control group [48.26] which decreased [P<0.001]. Anxiety scores at discharge decreased in the intervention group [33.24] compared with the control group [41.22] which was significant [P<0.001]. The mean patient satisfaction score at discharge in the intervention group [9.77] compared to control group [6.11] was statistically significance [P<0.001]. Orientation tour in patient candidates to angiography, reduces anxiety and increases satisfaction, therefore, use of this educational approach in patients undergoing coronary angiography is recommended

[Screening of mitochondrial mutations of A1555G, A 3243G, and A7445G in MTRNR1, MTTL1 and MTTS1 genes in subjects with nonsyndromic sensorineural hearing loss]

Various frequencies of the mtDNA mutations have been reported from different population world wild. Three mitochondrial DNA [mtDNA] mutations including A1555G, A 3243G, and A7445G which occurred in MTRNR1, MTTL1 and MTTS1 genes were considered as the main causes of mitochondrial hearing loss in some populations. To determine the frequency of the A1555G, A3243G, and A7445G mutations in nonsyndromic sensorineural hearing loss subjects in Gilan. Forty six subjects with nonsyndromic sensorineural hearing loss were screened by provided questionnaire and audiogram from Gillan Welfare Organization. PCR-RFLP procedure was used in order to presence the MtDNA of A1555GA 3243G and A7445G mutations and was confirmed by subsequent direct sequencing. There was no MtDNA of A1555G, A3243G and A7445G mutation in the cohort study of 46 deaf individuals. Investigation of PCR-RFLP of the MTTL1 gene for existence A3243G mutation lead to identification a G3316A variant that destroyed other restriction site, in the other site of PCR fragment. Our finding indicated that possibility the association of mitochondrial mutations with deafness is very low in deaf subjects in north of Iran. According to existence the G3316A that its pathogenesis in relation to hearing loss phenotype has not stabilized, the frequency of G3316A is 1.46% that can be had highlights role of mitochondrial mutation in deafness

[Frequency of 35delG mutation in GJB2 gene in non-syndromic prelingual hearing loss in 3 provinces of Iran]

Hearing loss is the most common inherited sensory disorder. At least 50% of hearing loss is inherited and about half of the genetic hearing loss is autosomal recessive non-syndromic. Mutations in GJB2 gene is the most frequent cause of autosomal recessive non-syndromic hearing loss. A single 35delG mutation is the most common allelic variant of GJB2 in most parts of the world. The aim of this study was to determine the rate of 35delG mutation in non-syndromic prelingual hearing loss in 3 provinces of Iran. In this descriptive experimental study, 240 cases with autosomal recessive non-syndromic hearing loss in 3 provinces of Iran, including Azarbaijan Sharghi [97 cases], Chaharmahal va Bakhtiari [98 cases] and Gilan [45 cases] were screened for 35delG mutation in the GJB2 gene. Blood samples [5 ml] were taken for genomic DNA extraction. The mutation was screened using Nested-PCR method and the positive results were confirmed by subsequent direct sequencing. Results of this study showed that from 240 studied patients [480 chromosomes], 35delG mutation was found in 58 chromosomes [24 patients were homozygote and 10 patients were heterozygote]. The frequency of 35delG mutation was 12.08%, including 18.04% in Azarbaijan Sharghi, 3.06% in Chaharmahal va Bakhtiari and 18.88% in Gilan province. Prevalence of 35delG mutation in Chaharmahal va Bakhtiari population was lower than other provinces studied. These results indicate that the other genes or mutations could result in autosomal recessive non-syndromic hearing loss in Chaharmahal va Bakhtiari population. However, as we found a low rate of 35delG in the populations studied, the cause of deafness remains to be detected in other loci or genes

[Comparison between therapeutic effects of doxepine and hydroxyzine on resistant pruritic lesions due to sulfur mustard]

Chronic pruritus is one of the major complaints in chemically-injured patients. It may cause many psychological problems and consequently, decrease the patient's quality of life. The aim of this study is to compare the safety and efficacy of Doxepin and Hydroxyzine in treatment of chronic pruritus due to Sulfur Mustard. This randomized, double-blind clinical trial was carried out in Baqiyatallah hospital on 50 chemically-injured patients for a period of 4 weeks. Patients randomly divided into 2 groups, first group [mean age of 42.3 +/- 5.4 years old] received Doxepin capsules [10 mg/day] and the second group [mean age of 41.1 +/- 6.2 years old] received Hydroxyzine capsules [25 mg/day]. Pruritus score was measured by a standard questionnaire and Visual Analogue Scale [VAS]. The mean before and after-treatment puritus scores of Hydroxyzine, were 34.6 +/- 3.4 and 25.9 +/- 3.1, respectively [P<0.001] and those of Doxepin were 33.8 +/- 4.4 and 24.5 +/- 4.1, respectively [P<0.001]. Both drugs decreased pruritus, similarly [P = 0.245]. 18 patients in the hydroxyzine-received group and 11 patients in the Doxepin-received group were complaining from sedation as a side-effect [P=0.035]. Both drugs are of significant effectiveness in decreasing pruritus. However, considering its fewer side effects, Doxepin seems to be more useful in these conditions

[ comparison of vitamin C and WBC in asthmatic and normal person]

Background: Vitamin C is a major antioxidant in lung airways and also functions against external insulting oxidant sources such as smoke and environmental contaminants. The aim of study was to assess the vitamin C in asthmatic patients, by it's measurement in plasma and white blood cells [WBC]

Materials and Methods: In a case-control study 50 asthmatic patients and 50 healthy person were randomly selected. The data were obtained by utilizing socioeconomic questionnaire and 24 hour dietary recall. 10 c.c blood samples were drawn. Plasma and WBC vitamin C concentration were measured colorimetrically. The data were analyzed by FPI and SPSS software

Results: The results indicate that 38 percent and 92 percent of patients had deficient plasma and WBC vitamin C concentrations respectively. There was a significant difference in plasma and WBC vitamin C concentrations among patients and control group [P<0.0001] Analysis of regression showed that a significant dependence was observed between the duration of asthma and plasma vitamin C status [P=0.03], though a positive significant correlation was obtained between plasma vitamin C status and dietary vitamin C intake [P=0.0001, r =0.56], The interaction of socioeconomic parameters and vitamin C concentration in asthmatic patients in this study was not significant

Conclusion: Present study indicates that while there is an association between vitamin C status and asthma but WBC vitamin C status is more sensitive in this regard and deserves further study and consideration